Tirzepatide 30mg dosage protocol is recognized for its once-weekly subcutaneous injection schedule, following a gradual escalation from 2.5 mg up to 15 mg weekly.
Initial dose: 2.5 mg once weekly for 4 weeks
Incrementally increase the dose every 4 weeks as tolerated
FDA-approved range up to 15 mg weekly
Reconstitute to a practical concentration for accurate dosing
Store lyophilized vials in the freezer; reconstituted solution in the refrigerator
Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard Titration Approach (3.0 mL = 10 mg/mL)
Phase (Weeks)
Weekly Dose (mg)
Units (per injection)
Weeks 1–4
2.5 mg
25 units (0.25 mL)
Weeks 5–8
5 mg
50 units (0.50 mL)
Weeks 9–12
7.5 mg
75 units (0.75 mL)
Weeks 13–16
10 mg
100 units (1.00 mL)
Weeks 17–20
12.5 mg
125 units (1.25 mL)
Weeks 21–24
15 mg
150 units (1.50 mL)
By adding 3.0 mL of bacteriostatic water, each vial yields a 10 mg/mL concentration, ensuring practical injection volumes (≥10 syringe units). This single 30 mg vial can provide multiple weekly doses, depending on your schedule.
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Slowly inject along the vial wall to minimize foaming.
Gently swirl—do not shake vigorously.
Label the vial (date/time) and store at 2–8°C.
Use within 30 days; avoid repeated freeze-thaw cycles.
If your dose calculation somehow drops below ~10 units even with 3.0 mL, consider smaller insulin syringes for more accurate dosing.
Protocol Overview
A concise summary of the weekly tirzepatide escalation.
Goal: Improve metabolic markers and support weight management
Schedule: Once-weekly subcutaneous injections, escalating in 2.5 mg increments
Dose Range: 2.5 mg to 15 mg weekly, depending on tolerance
Reconstitution: Up to 3.0 mL for a convenient 10 mg/mL solution
Storage: Keep lyophilized vials frozen; refrigerate after reconstitution
Dosing Protocol
Suggested once-weekly injection approach with a gradual titration phase.
Weekly Dose: Start at 2.5 mg for 4 weeks; increase by 2.5 mg every 4 weeks as tolerated
Frequency: Inject subcutaneously once per week
Cycle Length: Typically 12–24 weeks, or longer if needed
Maximum Dose: Up to 15 mg weekly after proper titration
Timing: Consistent weekly schedule
Storage Instructions
Proper storage preserves peptide integrity and potency.
Lyophilized: Freeze until ready to mix (−20°C)
Reconstituted: Refrigerate at 2–8°C
Use within 30 days after reconstitution
Avoid repeated freeze-thaw cycles
Supplies Needed
Have these items on hand for a 12–24+ week cycle.
Peptide Vials:
• 8 wks ≈ 1 vial
• 12 wks ≈ 1–2 vials
• 16 wks ≈ 2 vials
• 24 wks ≈ 2–3 vials
(Higher doses per week may require additional vials)
Insulin Syringes:
• One syringe per weekly injection
• Consider extras for dosage adjustments or multiple cycles
Bacteriostatic Water: 1× 30ml (for mixing as needed)
Alcohol Swabs: 1 box
Important Notes
Additional tips for a smooth research experience.
Use fresh, sterile insulin syringes and rotate injection sites weekly.
Adhere to the once-weekly schedule for consistent results.
Monitor for side effects; consult a professional if concerns arise.
Record doses and responses to guide future adjustments.
How This Works
Tirzepatide is a dual agonist that targets both GLP-1 and GIP receptors to support metabolic function.
Dual Hormone Pathway: Enhances insulin sensitivity and helps control appetite
Metabolic Benefits: Potentially aids in glucose regulation and body weight management
May offer improved glycemic control in research models
Potential Benefits & Side Effects
Most users observe improvements in metabolic parameters, but individual responses may vary.
May promote healthier blood glucose levels
Often supports weight management objectives
Common side effects include mild GI discomfort or nausea
Rarely, injection-site irritation or headaches could occur
Lifestyle Factors
Supportive habits can maximize the effectiveness of tirzepatide protocols.
Prioritize balanced, protein-rich nutrition
Engage in regular physical activity and allow adequate recovery
Manage stress and ensure sufficient sleep
Injection Technique
Simple guidelines for safe daily injections.
Clean vial rubber stopper & injection site with alcohol swabs
Insert needle at a 45–90° angle into subcutaneous tissue
